Human FSTL1 (Follistatin-related protein 1) Sandwich ELISA Kit (STJE0002031)

SPECIFICATIONS
STJE0002031
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General Information

Short DescriptionThis FSTL1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity0.19ng/mL
Detection Limit0.31~20ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolFSTL1
Gene ID11167
Uniprot IDFSTL1_HUMAN
SpecificityThis kit recognizes Human FSTL1 in samples. No significant cross-reactivity or interference between Human FSTL1 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation. Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes. Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors.
Protein Name Follistatin-Related Protein 1
Follistatin-Like Protein 1
Database Links Reactome: R-HSA-201451
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative ELISA Names Follistatin-Related Protein 1 ELISA kit
Follistatin-Like Protein 1 ELISA kit
FSTL1 ELISA kit
FRP ELISA kit
Specificity This kit recognizes Human FSTL1 in samples. No significant cross-reactivity or interference between Human FSTL1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-103 98
EDTA plasma (n=8) 93-107 100
Cell culture media (n=8) 89-104 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.05 1.82 8.30 1.05 1.92 8.30
Standard deviation 0.06 0.08 0.25 0.07 0.10 0.38
CV (%) 5.71 4.40 3.01 6.67 5.21 4.58

Citations

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