Human Frizzled-2/FZD2 protein (Recombinant) (C-hFc&His) (STJP001414)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Frizzled-2/FZD2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln24-Leu163) of human Frizzled-2/FZD2 (Accession #NP_001457.1) fused with a Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Frizzled-2/FZD2-C-hFc&His protein was developed in hek293 cells using the region Gln24-Leu163. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | FZD2 |
| Gene ID | 2535 |
| Uniprot ID | FZD2_HUMAN |
| Immunogen | Recombinant Human Frizzled-2/FZD2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln24-Leu163) of human Frizzled-2/FZD2 (Accession #NP_001457.1) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Gln24-Leu163 |
| Immunogen Sequence | QFHGEKGISIPDHGFCQPIS IPLCTDIAYNQTIMPNLLGH TNQEDAGLEVHQFYPLVKVQ CSPELRFFLCSMYAPVCTVL EQAIPPCRSICERARQGCEA LMNKFGFQWPERLRCEHFPR HGAEQICVGQNHSEDGAPAL |
Additional Info
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling. |
| Protein Name | Frizzled-2Fz-2Hfz2Fze2 |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-373080Reactome: R-HSA-4086398Reactome: R-HSA-4608870Reactome: R-HSA-4641262Reactome: R-HSA-5140745 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Protein Names | Frizzled-2 proteinFz-2 proteinHfz2 proteinFze2 proteinFZD2 protein |
Information sourced from Uniprot.org