Human FOXA2 (FOXA2) Antibody Pair (STJAP00774)
General Information
| Short Description | This FOXA2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R56-1R-9/R87-3V-7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | FOXA2 |
| Gene ID | 3170 |
| Uniprot ID | FOXA2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. |
| Function | Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-ACAATTAGTTGTAGCTTCT-3'. In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs.FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. |
| Protein Name | Hepatocyte Nuclear Factor 3-BetaHnf-3-BetaHnf-3bForkhead Box Protein A2Transcription Factor 3bTcf-3b |
| Database Links | Reactome: R-HSA-210745Reactome: R-HSA-9796292Reactome: R-HSA-9823730Reactome: R-HSA-9925561 |
| Cellular Localisation | NucleusCytoplasmShuttles Between The Nucleus And Cytoplasm In A Crm1-Dependent MannerIn Response To Insulin Signaling Via Akt1 Is Exported From The Nucleus |
| Alternative Antibody Pair Names | Hepatocyte Nuclear Factor 3-Beta antibody pairHnf-3-Beta antibody pairHnf-3b antibody pairForkhead Box Protein A2 antibody pairTranscription Factor 3b antibody pairTcf-3b antibody pairFOXA2 antibody pairHNF3B antibody pairTCF3B antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human FOXA2 (FOXA2) Antibody Pair (STJAP00774)
This antibody pair is designed to detect human FOXA2 (UniProt Q9Y261) and has confirmed reactivity in human samples only. Reactivity in other species has not been specified for this product.
This antibody pair has been validated for use in ELISA. It has not been listed as validated for other assay formats such as lateral flow or multiplex immunoassay.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. They are affinity purified and identified by clone IDs R56-1R-9 and R87-3V-7.
Optimal working dilutions for both antibodies are to be determined by the end user. Titration within the user's specific experimental system is recommended to establish appropriate concentrations.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies were raised against a recombinant protein immunogen corresponding to human FOXA2. No further detail on the specific immunogen region is provided in the product specification.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.