Human Follistatin protein (Recombinant) (STJP000469)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Follistatin mature chain was expressed inHek293 cells. NA
General Information
| Short Description | Recombinant-Human Follistatin-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised at 1mg/ml containing no additives. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) was determined by theability to neutralize Activin A inhibitory effect of mouse MPC-11 cells and was determined to be 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A. NA |
Target Information
| Gene Symbol | FST |
| Gene ID | 10468 |
| Uniprot ID | FST_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Follistatin mature chain was expressed inHek293 cells. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | NA |
Additional Info
| Tissue Specificity | Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid. In the embryo, strong expression is seen in the palatal epithelia, including the medial edge epithelial and midline epithelial seam of the palatal shelves. Less pronounced expression is also seen throughout the palatal shelf and tongue mesenchyme. |
| Function | Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling. Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin. Also promotes neural differentiation by antagonizing the action BMP4. Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA. On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA. |
| Protein Name | FollistatinFsActivin-Binding Protein |
| Database Links | Reactome: R-HSA-2473224 |
| Cellular Localisation | SecretedNucleusNucleolus |
| Alternative Protein Names | Follistatin proteinFs proteinActivin-Binding Protein proteinFST protein |
Information sourced from Uniprot.org