Human Follistatin/FST protein (Recombinant) (C-His) (STJP001262)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenActive Recombinant Human Follistatin/FST Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly30-Trp344) of human Follistatin (Accession #NP_037541.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Follistatin/FST-C-His protein was developed in hek293 cells using the region Gly30-Trp344. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Inhibin beta A at 0.5 Mu g/mL (100 Mu L/well) can bind recombinant Human FST, the EC50 of Human FST is 7.1 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | FST |
| Gene ID | 10468 |
| Uniprot ID | FST_HUMAN |
| Immunogen | Active Recombinant Human Follistatin/FST Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly30-Trp344) of human Follistatin (Accession #NP_037541.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Gly30-Trp344 |
| Immunogen Sequence | GNCWLRQAKNGRCQVLYKTE LSKEECCSTGRLSTSWTEED VNDNTLFKWMIFNGGAPNCI PCKETCENVDCGPGKKCRMN KKNKPRCVCAPDCSNITWKG PVCGLDGKTYRNECALLKAR CKEQPELEVQYQGRCKKTCR DVFCPGSSTCVVDQTNNAYC VTCNRICPEPASSEQYLCGN DGVTYSSACHLRKATCLLGR SIGLAYEGKCIKAKSCEDIQ CTGGKKCLWDFKVGRGRCS |
Additional Info
| Function | Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling. Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin. Also promotes neural differentiation by antagonizing the action BMP4. Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA. On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA. |
| Protein Name | FollistatinFsActivin-Binding Protein |
| Database Links | Reactome: R-HSA-2473224 |
| Cellular Localisation | SecretedNucleusNucleolus |
| Alternative Protein Names | Follistatin proteinFs proteinActivin-Binding Protein proteinFST protein |
Information sourced from Uniprot.org