• Human FLT3LG (Fms-related tyrosine kinase 3 ligand) CLIA Kit (STJC0000069)

Human FLT3LG (Fms-related tyrosine kinase 3 ligand) CLIA Kit (STJC0000069)

SKU:
STJC0000069

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human FLT3LG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: FLT3LG
Gene ID: 2323
Uniprot ID: FLT3L_HUMAN
Specificity: This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Protein Name Fms-Related Tyrosine Kinase 3 Ligand
Flt3 Ligand
Flt3l
Sl Cytokine
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-9607240
Reactome: R-HSA-9645135
Reactome: R-HSA-9706369
Reactome: R-HSA-9706374
Reactome: R-HSA-9706377
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative CLIA Names Fms-Related Tyrosine Kinase 3 Ligand CLIA kit
Flt3 Ligand CLIA kit
Flt3l CLIA kit
Sl Cytokine CLIA kit
FLT3LG CLIA kit
Specificity This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 98
EDTA plasma(n=8) 97-110 105
Cell culture media(n=8) 99-115 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 27.22 42.60 226.45 26.78 46.32 221.91
Standard deviation 2.21 4.17 25.29 3.21 5.49 13.36
CV (%) 8.12 9.79 11.17 11.99 11.85 6.02
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance