Human FLT3LG (Fms-related tyrosine kinase 3 ligand) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human FLT3LG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 4.69pg/mL |
| Detection Limit | 7.81~500pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | FLT3LG |
| Gene ID | 2323 |
| Uniprot ID | FLT3L_HUMAN |
| Specificity | This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs). |
| Protein Name | Fms-Related Tyrosine Kinase 3 LigandFlt3 LigandFlt3lSl Cytokine |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-9607240Reactome: R-HSA-9645135Reactome: R-HSA-9706369Reactome: R-HSA-9706374Reactome: R-HSA-9706377 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative CLIA Names | Fms-Related Tyrosine Kinase 3 Ligand CLIA kitFlt3 Ligand CLIA kitFlt3l CLIA kitSl Cytokine CLIA kitFLT3LG CLIA kit |
| Specificity | This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-107 | 98 |
| EDTA plasma(n=8) | 97-110 | 105 |
| Cell culture media(n=8) | 99-115 | 105 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 27.22 | 42.60 | 226.45 | 26.78 | 46.32 | 221.91 |
| Standard deviation | 2.21 | 4.17 | 25.29 | 3.21 | 5.49 | 13.36 |
| CV (%) | 8.12 | 9.79 | 11.17 | 11.99 | 11.85 | 6.02 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human FLT3LG (Fms-related tyrosine kinase 3 ligand) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect human FLT3LG (Fms-related tyrosine kinase 3 ligand) in serum, plasma, and other biological fluids. It is validated for human samples only and has no significant cross-reactivity with analogues of human Flt3L.
This kit has a detection range of 7.81 to 500 pg/mL and a sensitivity of 4.69 pg/mL.
This kit uses chemiluminescence (CLIA) as its detection method for quantitative measurement of human Flt3L.
The total assay time is approximately 3.5 hours from start to finish.
If unopened, this kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month, components should be stored separately according to the conditions specified in the manual.
No significant cross-reactivity or interference between human Flt3L and its analogues was observed, indicating the assay is specific to the target analyte in human samples.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.