| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Fibronectin/CIG/FN1-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin at 1 Mu g/mL (100 Mu L/well) can bind Fibronectin Rabbit mAb with a linear range of 0.1-0.89 ng/mL. |
| Gene Symbol: |
FN1 |
| Gene ID: |
2335 |
| Uniprot ID: |
FINC_HUMAN |
| Immunogen: |
Recombinant Human Fibronectin/CIG/FN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser607-Pro1265) of human Fibronectin (Accession #NP_997647.1) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. |
| Protein Name | FibronectinFnCold-Insoluble GlobulinCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1474228Reactome: R-HSA-1474244Reactome: R-HSA-1566977Reactome: R-HSA-202733Reactome: R-HSA-2129379Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381426Reactome: R-HSA-5674135Reactome: R-HSA-6785807Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8874081Reactome: R-HSA-8957275Reactome: R-HSA-9634597Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Fibronectin proteinFn proteinCold-Insoluble Globulin proteinCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 proteinFN1 proteinFN protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
