Human Fibronectin/CIG/FN1 protein (Recombinant) (C-His) (STJP001408)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Fibronectin/CIG/FN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn1722-Thr1811) of human Fibronectin (Accession #NP_997647.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Fibronectin/CIG/FN1-C-His protein was developed in hek293 cells using the region Asn1722-Thr1811. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When cells are added to Fibronectin coated plates (2.5 Mu g/mL and 100 Mu L/well) , approximately 30%-40% cells will adhere specifi |
| Determination Method | Please contact us for more information. |
Target Information
| Gene Symbol | FN1 |
| Gene ID | 2335 |
| Uniprot ID | FINC_HUMAN |
| Immunogen | Recombinant Human Fibronectin/CIG/FN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn1722-Thr1811) of human Fibronectin (Accession #NP_997647.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Asn1722-Thr1811 |
| Immunogen Sequence | NIDRPKGLAFTDVDVDSIKI AWESPQGQVSRYRVTYSSPE DGIHELFPAPDGEEDTAELQ GLRPGSEYTVSVVALHDDME SQPLIGTQST |
Additional Info
| Post Translational Modifications | Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. |
| Protein Name | FibronectinFnCold-Insoluble GlobulinCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1474228Reactome: R-HSA-1474244Reactome: R-HSA-1566977Reactome: R-HSA-202733Reactome: R-HSA-2129379Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381426Reactome: R-HSA-5674135Reactome: R-HSA-6785807Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8874081Reactome: R-HSA-8957275Reactome: R-HSA-9634597Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9860927 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Fibronectin proteinFn proteinCold-Insoluble Globulin proteinCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 proteinFN1 proteinFN protein |
Information sourced from Uniprot.org