Human FGF21 (Fibroblast growth factor 21) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human FGF21 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 7.5pg/mL |
| Detection Limit | 12.5~800pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | FGF21 |
| Gene ID | 26291 |
| Uniprot ID | FGF21_HUMAN |
| Specificity | This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity. |
| Protein Name | Fibroblast Growth Factor 21Fgf-21 |
| Database Links | Reactome: R-HSA-189200Reactome: R-HSA-8963889 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Fibroblast Growth Factor 21 CLIA kitFgf-21 CLIA kitFGF21 CLIA kitUNQ3115 CLIA kitPRO10196 CLIA kit |
| Specificity | This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-99 | 92 |
| EDTA plasma(n=8) | 99-113 | 106 |
| Cell culture media(n=8) | 97-113 | 104 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 39.32 | 81.85 | 297.58 | 39.70 | 80.40 | 316.22 |
| Standard deviation | 4.68 | 7.07 | 23.48 | 3.50 | 7.26 | 28.62 |
| CV (%) | 11.90 | 8.64 | 7.89 | 8.82 | 9.03 | 9.05 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human FGF21 (Fibroblast growth factor 21) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect Human FGF21 (Fibroblast growth factor 21, UniProt Q9NSA1) in human serum, plasma, and other biological fluids. No significant cross-reactivity or interference with FGF21 analogues was observed.
This kit has a detection range of 12.5 to 800 pg/mL and a sensitivity of 7.5 pg/mL.
This kit uses chemiluminescence (CLIA) for quantitative detection of Human FGF21.
The total assay time is 3.5 hours from start to finish.
If unopened, this kit may be stored at 2 to 8 degrees Celsius for up to 1 month. If the kit will not be used within 1 month, components should be stored separately according to the component table provided in the manual.
No, this kit is intended for research use only and is not approved for diagnostic or therapeutic applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.