Human FGF2 (FGF2) Antibody Pair (STJAP00650)
General Information
| Short Description | This FGF2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 4G11/3D1 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | FGF2 |
| Gene ID | 2247 |
| Uniprot ID | FGF2_HUMAN |
| Immunogen | A synthesized peptide derived from human bFGF |
Additional Info
| Tissue Specificity | Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. |
| Post Translational Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
| Function | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. |
| Protein Name | Fibroblast Growth Factor 2Fgf-2Basic Fibroblast Growth FactorBfgfHeparin-Binding Growth Factor 2Hbgf-2 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-1839122Reactome: R-HSA-1839130Reactome: R-HSA-190322Reactome: R-HSA-190370Reactome: R-HSA-190372Reactome: R-HSA-190373Reactome: R-HSA-190375Reactome: R-HSA-190377Reactome: R-HSA-2033519Reactome: R-HSA-2219530Reactome: R-HSA-2892247Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-5654219Reactome: R-HSA-5654221Reactome: R-HSA-5654227Reactome: R-HSA-5654228Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654695Reactome: R-HSA-5654699Reactome: R-HSA-5654700Reactome: R-HSA-5654704Reactome: R-HSA-5654706Reactome: R-HSA-5654710Reactome: R-HSA-5654712Reactome: R-HSA-5654719Reactome: R-HSA-5654720Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5655253Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5658623Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6811558Reactome: R-HSA-8851708Reactome: R-HSA-9761174Reactome: R-HSA-9830364Reactome: R-HSA-9839397Reactome: R-HSA-9925561 |
| Cellular Localisation | SecretedNucleusExported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent MechanismUnconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma MembraneBinding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Cep57 |
| Alternative Antibody Pair Names | Fibroblast Growth Factor 2 antibody pairFgf-2 antibody pairBasic Fibroblast Growth Factor antibody pairBfgf antibody pairHeparin-Binding Growth Factor 2 antibody pairHbgf-2 antibody pairFGF2 antibody pairFGFB antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human FGF2 (FGF2) Antibody Pair (STJAP00650)
This antibody pair detects human FGF2 (basic fibroblast growth factor), corresponding to UniProt entry P09038. Reactivity has been confirmed for human samples only.
Both antibodies are mouse monoclonal IgG1 antibodies supplied as affinity-purified liquids at 1 mg/mL. The capture antibody (clone 4G11) is formulated in PBS with 0.09% sodium azide, and the detection antibody (clone 3D1) is formulated in PBS with 0.01% thimerosal. Neither antibody carries a conjugate or tag.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
A dilution of 1:1000 is recommended for ELISA. Researchers may need to optimize conditions empirically depending on their specific experimental setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Both antibodies were raised against a synthesized peptide derived from human bFGF. Both clones are hosted in mouse and were selected as a matched pair for sandwich ELISA detection of the human FGF2 protein.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.