Human FGF1 (FGF1) Antibody Pair (STJAP00963)
General Information
| Short Description | This FGF1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | FGF1 |
| Gene ID | 2246 |
| Uniprot ID | FGF1_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | In the nucleus, phosphorylated by PKC/PRKCD. |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis. |
| Protein Name | Fibroblast Growth Factor 1Fgf-1Acidic Fibroblast Growth FactorAfgfEndothelial Cell Growth FactorEcgfHeparin-Binding Growth Factor 1Hbgf-1 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-1839122Reactome: R-HSA-1839130Reactome: R-HSA-190322Reactome: R-HSA-190370Reactome: R-HSA-190371Reactome: R-HSA-190372Reactome: R-HSA-190373Reactome: R-HSA-190375Reactome: R-HSA-190377Reactome: R-HSA-2033519Reactome: R-HSA-2219530Reactome: R-HSA-5654219Reactome: R-HSA-5654221Reactome: R-HSA-5654227Reactome: R-HSA-5654228Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654695Reactome: R-HSA-5654699Reactome: R-HSA-5654700Reactome: R-HSA-5654704Reactome: R-HSA-5654706Reactome: R-HSA-5654710Reactome: R-HSA-5654712Reactome: R-HSA-5654719Reactome: R-HSA-5654720Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5655253Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-8851708 |
| Cellular Localisation | SecretedCytoplasmCell CortexCytosolNucleusLacks A Cleavable Signal SequenceWithin The CytoplasmIt Is Transported To The Cell Membrane And Then Secreted By A Non-Classical Pathway That Requires Cu(2+) Ions And S100a13Secreted In A Complex With Syt1Binding Of Exogenous Fgf1 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf1 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Lrrc59 |
| Alternative Antibody Pair Names | Fibroblast Growth Factor 1 antibody pairFgf-1 antibody pairAcidic Fibroblast Growth Factor antibody pairAfgf antibody pairEndothelial Cell Growth Factor antibody pairEcgf antibody pairHeparin-Binding Growth Factor 1 antibody pairHbgf-1 antibody pairFGF1 antibody pairFGFA antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human FGF1 (FGF1) Antibody Pair (STJAP00963)
This antibody pair detects human FGF1 (Fibroblast Growth Factor 1, UniProt P05230) and is validated for reactivity with human samples only.
The capture antibody is supplied unconjugated at 0.5 mg/mL, and the detection antibody is supplied biotinylated at a concentration of 0.5 to 4 ug/mL. Both antibodies are raised in goat.
This pair has been validated for use in sandwich ELISA with colorimetric detection. It has not been validated for other assay formats such as lateral flow or multiplex.
This pair is suitable for use with serum, plasma, and other biological fluids in a sandwich ELISA format.
A dilution range of 1:1000 to 1:10000 is recommended, though optimal working dilutions should be determined empirically for each specific experimental setup.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.