Human FGF-2/bFGF protein (Recombinant) (No tag) (STJP001122)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenActive Recombinant Human FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro143-Ser288) of human FGF2 (Accession #NP_001997.5).
General Information
| Short Description | Recombinant-Human FGF-2/bFGF-No tag protein was developed in e.coli using the region Pro143-Ser288. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150 mM NaCl, pH 7.5. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 0.5 Mu g/mL (100 Mu L/well) can bind Human GPC3 with a linear range of 7-20 ng/mL.|2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblast |
| Determination Method | < 1.0 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | FGF2 |
| Gene ID | 2247 |
| Uniprot ID | FGF2_HUMAN |
| Immunogen | Active Recombinant Human FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro143-Ser288) of human FGF2 (Accession #NP_001997.5). |
| Immunogen Region | Pro143-Ser288 |
| Immunogen Sequence | PALPEDGGSGAFPPGHFKDP KRLYCKNGGFFLRIHPDGRV DGVREKSDPHIKLQLQAEER GVVSIKGVCANRYLAMKEDG RLLASKCVTDECFFFERLES NNYNTYRSRKYTSWYVALKR TGQYKLGSKTGPGQKAILFL PMSAKS |
Additional Info
| Post Translational Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
| Function | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. |
| Protein Name | Fibroblast Growth Factor 2Fgf-2Basic Fibroblast Growth FactorBfgfHeparin-Binding Growth Factor 2Hbgf-2 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-1839122Reactome: R-HSA-1839130Reactome: R-HSA-190322Reactome: R-HSA-190370Reactome: R-HSA-190372Reactome: R-HSA-190373Reactome: R-HSA-190375Reactome: R-HSA-190377Reactome: R-HSA-2033519Reactome: R-HSA-2219530Reactome: R-HSA-2892247Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-5654219Reactome: R-HSA-5654221Reactome: R-HSA-5654227Reactome: R-HSA-5654228Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654695Reactome: R-HSA-5654699Reactome: R-HSA-5654700Reactome: R-HSA-5654704Reactome: R-HSA-5654706Reactome: R-HSA-5654710Reactome: R-HSA-5654712Reactome: R-HSA-5654719Reactome: R-HSA-5654720Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5655253Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5658623Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6811558Reactome: R-HSA-8851708Reactome: R-HSA-9761174Reactome: R-HSA-9830364Reactome: R-HSA-9839397Reactome: R-HSA-9925561 |
| Cellular Localisation | SecretedNucleusExported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent MechanismUnconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma MembraneBinding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Cep57 |
| Alternative Protein Names | Fibroblast Growth Factor 2 proteinFgf-2 proteinBasic Fibroblast Growth Factor proteinBfgf proteinHeparin-Binding Growth Factor 2 proteinHbgf-2 proteinFGF2 proteinFGFB protein |
Information sourced from Uniprot.org