• Biotinylated Human Fc gamma RIIIA (V176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 2ng/ml determined by ELISA.
  • Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176) , His-Avi Tag with an affinity constant of 0. 539 Mu M as determined in SPR assay (Biacore T200).

Human Fc gamma RIIIA/CD16a (V176) protein (Recombinant) (C-His-Avi) {Biotin} (STJP001636)

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STJP001636

€773.13 - €2,386.88
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Fc gamma RIIIA/CD16a (V176)-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Immunogen Region: Gly17-Gln208
Immunogen: DNA sequence encoding Human Fc gamma RIIIA/CD16a (V176) including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance