Human FASLG (Tumor necrosis factor ligand superfamily member 6) Sandwich ELISA Kit (STJE0001964)

Name: Human FASLG (Tumor necrosis factor ligand superfamily member 6) Sandwich ELISA Kit (STJE0001964)
SKU: STJE0001964
Availability: InStock

⇓ Datasheet ⇓ SDS Citations

SPECIFICATIONS

STJE0001964

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General Information

Short Description	This FASLG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	FASLG
Gene ID	356
Uniprot ID	TNFL6_HUMAN
Specificity	This kit recognizes Human FASL/TNFSF6 in samples. No significant cross-reactivity or interference between Human FASL/TNFSF6 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Function	Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance. Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis. Tumor necrosis factor ligand superfamily member 6, soluble form: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways. Can induce apoptosis but does not appear to be essential for this process. FasL intracellular domain: Cytoplasmic form induces gene transcription inhibition.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 6 Apoptosis Antigen Ligand Aptl Cd95 Ligand Cd95-L Fas Antigen Ligand Fas Ligand Fasl Cd Antigen Cd178 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 6 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 6 - Soluble Form Receptor-Binding Fasl Ectodomain Soluble Fas Ligand Sfasl - Adam10-Processed Fasl Form Apl - Fasl Intracellular Domain Fasl Icd Sppl2a-Processed Fasl Form Spa
Database Links	Reactome: R-HSA-140534 Reactome: R-HSA-3371378 Reactome: R-HSA-5213460 Reactome: R-HSA-5218900 Reactome: R-HSA-6785807 Reactome: R-HSA-69416 Reactome: R-HSA-75157 Reactome: R-HSA-8862803 Reactome: R-HSA-9614657
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Cytoplasmic Vesicle Lumen Lysosome Lumen Is Internalized Into Multivesicular Bodies Of Secretory Lysosomes After Phosphorylation By Fgr And Monoubiquitination Colocalizes With The Sppl2a Protease At The Cell Membrane Tumor Necrosis Factor Ligand Superfamily Member 6 Soluble Form: Secreted May Be Released Into The Extracellular Fluid By Cleavage From The Cell Surface Fasl Intracellular Domain: Nucleus The Fasl Icd Cytoplasmic Form Is Translocated Into The Nucleus
Alternative ELISA Names	Tumor Necrosis Factor Ligand Superfamily Member 6 ELISA kit Apoptosis Antigen Ligand ELISA kit Aptl ELISA kit Cd95 Ligand ELISA kit Cd95-L ELISA kit Fas Antigen Ligand ELISA kit Fas Ligand ELISA kit Fasl ELISA kit Cd Antigen Cd178 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 6 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 6 - Soluble Form ELISA kit Receptor-Binding Fasl Ectodomain ELISA kit Soluble Fas Ligand ELISA kit Sfasl - Adam10-Processed Fasl Form ELISA kit Apl - Fasl Intracellular Domain ELISA kit Fasl Icd ELISA kit Sppl2a-Processed Fasl Form ELISA kit Spa ELISA kit FASLG ELISA kit APT1LG1 ELISA kit CD95L ELISA kit FASL ELISA kit TNFSF6 ELISA kit
Specificity	This kit recognizes Human FASL/TNFSF6 in samples. No significant cross-reactivity or interference between Human FASL/TNFSF6 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-108	102
EDTA plasma (n=8)	93-109	100
Cell culture media (n=8)	87-99	94

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	50.15	142.30	454.75	50.49	135.64	412.66
Standard deviation	2.94	8.24	14.92	2.90	6.47	15.76
CV (%)	5.86	5.79	3.28	5.74	4.77	3.82

Citations

View product citations for antibody STJE0001964 on CiteAb