Human Fas ligand/APTL/CD95 ligand/CD178 protein (Recombinant) (N-His) (STJP002146)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Fas ligand/APTL/CD95 ligand/CD178 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Pro134-Leu281) of Human Fas Ligand (monomer) fused with a His tag at the N-terminal.
General Information
| Short Description | Recombinant-Human Fas ligand/APTL/CD95 ligand/CD178-N-His protein was developed in hek293 cells using the region Pro134-Leu281. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 2.075-8.3 ng/mL in the presence of 10 µg/mL of a cross-linking antibody Rabbit Anti-poly Histidine Monoclonal Antibody, co |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | FASLG |
| Gene ID | 356 |
| Uniprot ID | TNFL6_HUMAN |
| Immunogen | Recombinant Human Fas ligand/APTL/CD95 ligand/CD178 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Pro134-Leu281) of Human Fas Ligand (monomer) fused with a His tag at the N-terminal. |
| Immunogen Region | Pro134-Leu281 |
| Immunogen Sequence | PSPPPEKKELRKVAHLTGKS NSRSMPLEWEDTYGIVLLSG VKYKKGGLVINETGLYFVYS KVYFRGQSCNNLPLSHKVYM RNSKYPQDLVMMEGKMMSYC TTGQMWARSSYLGAVFNLTS ADHLYVNVSELSLVNFEESQ TFFGLYKL |
Additional Info
| Function | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance. Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis. Tumor necrosis factor ligand superfamily member 6, soluble form: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways. Can induce apoptosis but does not appear to be essential for this process. FasL intracellular domain: Cytoplasmic form induces gene transcription inhibition. |
| Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 6Apoptosis Antigen LigandAptlCd95 LigandCd95-LFas Antigen LigandFas LigandFaslCd Antigen Cd178 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 6 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 6 - Soluble FormReceptor-Binding Fasl EctodomainSoluble Fas LigandSfasl - Adam10-Processed Fasl FormApl - Fasl Intracellular DomainFasl IcdSppl2a-Processed Fasl FormSpa |
| Database Links | Reactome: R-HSA-140534Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-6785807Reactome: R-HSA-69416Reactome: R-HSA-75157Reactome: R-HSA-8862803Reactome: R-HSA-9614657 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinCytoplasmic Vesicle LumenLysosome LumenIs Internalized Into Multivesicular Bodies Of Secretory Lysosomes After Phosphorylation By Fgr And MonoubiquitinationColocalizes With The Sppl2a Protease At The Cell MembraneTumor Necrosis Factor Ligand Superfamily Member 6Soluble Form: SecretedMay Be Released Into The Extracellular Fluid By Cleavage From The Cell SurfaceFasl Intracellular Domain: NucleusThe Fasl Icd Cytoplasmic Form Is Translocated Into The Nucleus |
| Alternative Protein Names | Tumor Necrosis Factor Ligand Superfamily Member 6 proteinApoptosis Antigen Ligand proteinAptl proteinCd95 Ligand proteinCd95-L proteinFas Antigen Ligand proteinFas Ligand proteinFasl proteinCd Antigen Cd178 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 6 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 6 - Soluble Form proteinReceptor-Binding Fasl Ectodomain proteinSoluble Fas Ligand proteinSfasl - Adam10-Processed Fasl Form proteinApl - Fasl Intracellular Domain proteinFasl Icd proteinSppl2a-Processed Fasl Form proteinSpa proteinFASLG proteinAPT1LG1 proteinCD95L proteinFASL proteinTNFSF6 protein |
Information sourced from Uniprot.org