Human FAP Alpha (Prolyl Endopeptidase Fap) Antibody Pair (STJAP00395)
SPECIFICATIONS
HostMouse/Sheep
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human FAP Alpha protien.Detection Immunogen: Recombinant Human FAP Alpha protien.
General Information
| Short Description | This FAP Alpha antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Sheep |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | FAP |
| Gene ID | 2191 |
| Uniprot ID | SEPR_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human FAP Alpha protien.Detection Immunogen: Recombinant Human FAP Alpha protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. The N-terminus may be blocked. |
| Function | Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein. Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner. |
| Protein Name | Prolyl Endopeptidase Fap170 Kda Melanoma Membrane-Bound GelatinaseDipeptidyl Peptidase FapFibroblast Activation Protein AlphaFapalphaGelatine Degradation Protease FapIntegral Membrane Serine ProteasePost-Proline Cleaving EnzymeSerine Integral Membrane ProteaseSimpSurface-Expressed ProteaseSeprase Cleaved Into - Antiplasmin-Cleaving Enzyme Fap - Soluble FormApce |
| Database Links | |
| Cellular Localisation | Prolyl Endopeptidase Fap: Cell SurfaceCell MembraneSingle-Pass Type Ii Membrane ProteinCell ProjectionLamellipodium MembraneInvadopodium MembraneRuffle MembraneMembraneLocalized On Cell Surface With Lamellipodia And Invadopodia Membranes And On Shed VesiclesColocalized With Dpp4 At Invadopodia And Lamellipodia Membranes Of Migratory Activated Endothelial Cells In Collagenous MatrixColocalized With Dpp4 On Endothelial Cells Of Capillary-Like Microvessels But Not Large Vessels Within Invasive Breast Ductal CarcinomaAnchored And Enriched Preferentially By Integrin Alpha-3/Beta-1 At InvadopodiaPlasma Membrane Protrusions That Correspond To Sites Of Cell InvasionIn A Collagen-Dependent MannerLocalized At Plasma And Ruffle Membranes In A Collagen-Independent MannerColocalized With Plaur Preferentially At The Cell Surface Of Invadopodia Membranes In A Cytoskeleton-Integrin- And Vitronectin-Dependent MannerConcentrated At Invadopodia MembranesSpecialized Protrusions Of The Ventral Plasma Membrane In A Fibrobectin-Dependent MannerColocalizes With Extracellular Components (Ecm)Such As Collagen Fibers And FibronectinAntiplasmin-Cleaving Enzyme FapSoluble Form: SecretedFound In Blood Plasma And SerumIsoform 2: Cytoplasm |
| Alternative Antibody Pair Names | Prolyl Endopeptidase Fap antibody pair170 Kda Melanoma Membrane-Bound Gelatinase antibody pairDipeptidyl Peptidase Fap antibody pairFibroblast Activation Protein Alpha antibody pairFapalpha antibody pairGelatine Degradation Protease Fap antibody pairIntegral Membrane Serine Protease antibody pairPost-Proline Cleaving Enzyme antibody pairSerine Integral Membrane Protease antibody pairSimp antibody pairSurface-Expressed Protease antibody pairSeprase Cleaved Into - Antiplasmin-Cleaving Enzyme Fap - Soluble Form antibody pairApce antibody pairFAP antibody pair |
Information sourced from Uniprot.org