Human Factor B (Factor B) Antibody Pair (STJAP00649)
General Information
| Short Description | This Factor B antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 1E4/5E1 |
| Isotype | IgG2b/IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CFB |
| Gene ID | 629 |
| Uniprot ID | CFAB_HUMAN |
| Immunogen | A synthesized peptide derived from human factor B |
Additional Info
| Post Translational Modifications | Cleaved by CFD following activation of the alternative complement system, generating Ba and Bb chains. Cleavage and activation takes place when CFB is already associated with complement C3b. |
| Function | Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases. CFB is cleaved and activated by CFD to generate Ba and Bb chains.Bb chain constituting the catalytic component of the C3 and C5 convertases. Complement factor B Bb: Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway. Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b. As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways. When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex. Complement factor B Ba: Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. |
| Protein Name | Complement Factor BC3/C5 ConvertaseGlycine-Rich Beta GlycoproteinGbgPbf2Properdin Factor B Cleaved Into - Complement Factor B BaBa - Complement Factor B BbBb |
| Database Links | Reactome: R-HSA-173736Reactome: R-HSA-174577Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedComplement Factor B Bb: Cell SurfaceRecruited To The Surface Of Pathogens By Complement C3b Opsonin |
| Alternative Antibody Pair Names | Complement Factor B antibody pairC3/C5 Convertase antibody pairGlycine-Rich Beta Glycoprotein antibody pairGbg antibody pairPbf2 antibody pairProperdin Factor B Cleaved Into - Complement Factor B Ba antibody pairBa - Complement Factor B Bb antibody pairBb antibody pairCFB antibody pairBF antibody pairBFD antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Factor B (Factor B) Antibody Pair (STJAP00649)
This antibody pair detects human Factor B (UniProt P00751) and has confirmed reactivity against human samples only. Both antibodies were raised against a synthesized peptide derived from human Factor B.
Both antibodies are supplied as affinity-purified mouse monoclonal IgGs at 1 mg/mL in liquid format. The clone 1E4 antibody (IgG2b) is formulated in PBS with 0.09% sodium azide, while the clone 5E1 antibody (IgG1) is formulated in PBS with 0.01% thimerosal.
This pair has been validated for use in ELISA at a dilution of 1:1000. No other assay formats are documented for this product.
Both antibodies are supplied unconjugated. Researchers requiring a labeled detection antibody will need to conjugate the relevant clone to their chosen reporter using standard antibody labeling methods.
Both antibodies should be stored at 4°C for short-term use and aliquoted and stored at -20°C for long-term storage. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.