Human F9 (Coagulation factor IX) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human F9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 0.19ng/mL |
| Detection Limit | 0.31~20ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | F9 |
| Gene ID | 2158 |
| Uniprot ID | FA9_HUMAN |
| Specificity | This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Detected in blood plasma (at protein level). Synthesized primarily in the liver and secreted in plasma. |
| Post Translational Modifications | Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. Activated by coagulation factor VIIa-tissue factor (F7-F3) complex in calcium-dependent manner. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. |
| Protein Name | Coagulation Factor IxChristmas FactorPlasma Thromboplastin ComponentPtc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain |
| Database Links | Reactome: R-HSA-140834Reactome: R-HSA-140837Reactome: R-HSA-159740Reactome: R-HSA-159763Reactome: R-HSA-159782Reactome: R-HSA-9629569Reactome: R-HSA-9672383Reactome: R-HSA-9672396Reactome: R-HSA-9673202Reactome: R-HSA-9673218Reactome: R-HSA-9673221Reactome: R-HSA-9673240 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Coagulation Factor Ix CLIA kitChristmas Factor CLIA kitPlasma Thromboplastin Component CLIA kitPtc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain CLIA kitF9 CLIA kit |
| Specificity | This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-107 | 99 |
| EDTA plasma(n=8) | 84-98 | 91 |
| Cell culture media(n=8) | 93-109 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.96 | 1.99 | 8.48 | 0.97 | 2.09 | 8.92 |
| Standard deviation | 0.11 | 0.20 | 0.68 | 0.11 | 0.18 | 1.01 |
| CV (%) | 11.46 | 10.05 | 8.02 | 11.34 | 8.61 | 11.32 |