Human F7 (Coagulation Factor Vii) Antibody Pair (STJAP00510)
General Information
| Short Description | This F7 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | F7 |
| Gene ID | 2155 |
| Uniprot ID | FA7_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human F7 protien.Detection Immunogen: Recombinant Human F7 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-S/T-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Can be either O-glucosylated or O-xylosylated at Ser-112 by POGLUT1 in vitro. |
| Function | Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium. |
| Protein Name | Coagulation Factor ViiProconvertinSerum Prothrombin Conversion AcceleratorSpcaEptacog Alfa Cleaved Into - Factor Vii Light Chain - Factor Vii Heavy Chain |
| Database Links | Reactome: R-HSA-1368108Reactome: R-HSA-140834Reactome: R-HSA-159740Reactome: R-HSA-159763Reactome: R-HSA-159782 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Coagulation Factor Vii antibody pairProconvertin antibody pairSerum Prothrombin Conversion Accelerator antibody pairSpca antibody pairEptacog Alfa Cleaved Into - Factor Vii Light Chain - Factor Vii Heavy Chain antibody pairF7 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human F7 (Coagulation Factor Vii) Antibody Pair (STJAP00510)
This antibody pair detects human Coagulation Factor VII (F7, UniProt P08709) and has confirmed reactivity with human samples only. Both the capture and detection antibodies were raised against recombinant human F7 protein.
This antibody pair is validated for use in ELISA and is suitable for measuring F7 in serum, plasma, and other biological fluids. No other assay formats are listed for this product.
Both antibodies are goat-derived and purified by antigen affinity, each supplied at 0.5 mg/mL. The capture antibody is unconjugated, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both the capture and detection antibodies should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
This antibody pair is intended for use with human serum, plasma, and other biological fluids in a sandwich ELISA format. It is for research use only and is not intended for diagnostic or therapeutic applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.