Human ES (Endostatin) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human ES kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 37.5pg/mL |
| Detection Limit | 62.5~4000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Specificity | This kit recognizes Human ES in samples. No significant cross-reactivity or interference between Human ES and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Specificity | This kit recognizes Human ES in samples. No significant cross-reactivity or interference between Human ES and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-98 | 93 |
| EDTA plasma(n=8) | 90-107 | 98 |
| Cell culture media(n=8) | 97-113 | 105 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 185.72 | 339.47 | 1770.97 | 193.38 | 345.78 | 1802.39 |
| Standard deviation | 23.49 | 29.70 | 142.03 | 16.42 | 26.52 | 140.41 |
| CV (%) | 12.65 | 8.75 | 8.02 | 8.49 | 7.67 | 7.79 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human ES (Endostatin) CLIA Kit for Quantitative Detection
This kit is designed for the quantitative detection of Human Endostatin (ES) in serum, plasma, and other biological fluids. It is validated for human samples only, and no significant cross-reactivity or interference with analogues was observed.
The detection range is 62.5 to 4000 pg/mL, with a sensitivity of 37.5 pg/mL. Samples with Endostatin concentrations outside this range may require dilution or concentration prior to testing.
This kit uses chemiluminescence (CLIA) as its detection method, which is reflected in the product classification as a Chemiluminescent Immunoassay kit.
The total assay time is approximately 3.5 hours from start to finish.
Unopened, the kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month, the components should be stored separately according to the storage conditions specified in the product manual.
No. This kit is intended for research use only and is not approved or validated for diagnostic, therapeutic, or clinical applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.