Human Erythropoietin/EPO protein (Recombinant) (No-Tag) (STJP002889)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Erythropoietin/EPO Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala28-Arg193) of human Erythropoietin/EPO (Accession #NP_000790.2) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Human Erythropoietin/EPO-No-Tag protein was developed in hek293 cells using the region Ala28-Arg193. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.07-0.27 ng/mL, corresponding to a specific activity of 3.70 × 106~1.43× 107 units/mg. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | EPO |
| Gene ID | 2056 |
| Uniprot ID | EPO_HUMAN |
| Immunogen | Recombinant Human Erythropoietin/EPO Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala28-Arg193) of human Erythropoietin/EPO (Accession #NP_000790.2) fused with no additional amino acid. |
| Immunogen Region | Ala28-Arg193 |
| Immunogen Sequence | APPRLICDSRVLERYLLEAK EAENITTGCAEHCSLNENIT VPDTKVNFYAWKRMEVGQQA VEVWQGLALLSEAVLRGQAL LVNSSQPWEPLQLHVDKAVS GLRSLTTLLRALGAQKEAIS PPDAASAAPLRTITADTFRK LFRVYSNFLRGKLKLYTGEA CRTGDR |
Additional Info
| Function | Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. |
| Protein Name | ErythropoietinEpoetin |
| Database Links | Reactome: R-HSA-1234158Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Erythropoietin proteinEpoetin proteinEPO protein |
Information sourced from Uniprot.org