Human ErbB2/HER2 (ErbB2/HER2) Antibody Pair (STJAP00849)
General Information
| Short Description | This ErbB2/HER2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R03-2Z-3/R09-6Y-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 2-8°C. Do not freeze. |
Target Information
| Gene Symbol | ERBB2 |
| Gene ID | 2064 |
| Uniprot ID | ERBB2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. |
| Post Translational Modifications | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12. |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. |
| Protein Name | Receptor Tyrosine-Protein Kinase Erbb-2Metastatic Lymph Node Gene 19 ProteinMln 19Proto-Oncogene NeuProto-Oncogene C-Erbb-2Tyrosine Kinase-Type Cell Surface Receptor Her2P185erbb2Cd Antigen Cd340 |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-1306955Reactome: R-HSA-1358803Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-2219530Reactome: R-HSA-416572Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8863795Reactome: R-HSA-8866910Reactome: R-HSA-9634285Reactome: R-HSA-9652282Reactome: R-HSA-9664565Reactome: R-HSA-9665233Reactome: R-HSA-9665244Reactome: R-HSA-9665245Reactome: R-HSA-9665246Reactome: R-HSA-9665247Reactome: R-HSA-9665249Reactome: R-HSA-9665250Reactome: R-HSA-9665251Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9665737 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionRuffle MembraneInternalized From The Cell Membrane In Response To Egf StimulationIsoform 1: Cell MembraneEarly EndosomeCytoplasmPerinuclear RegionNucleusTranslocation To The Nucleus Requires EndocytosisProbably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1Also Detected In Vps35-Positive Endosome-To-Tgn Retrograde VesiclesIsoform 2: CytoplasmIsoform 3: Cytoplasm |
| Alternative Antibody Pair Names | Receptor Tyrosine-Protein Kinase Erbb-2 antibody pairMetastatic Lymph Node Gene 19 Protein antibody pairMln 19 antibody pairProto-Oncogene Neu antibody pairProto-Oncogene C-Erbb-2 antibody pairTyrosine Kinase-Type Cell Surface Receptor Her2 antibody pairP185erbb2 antibody pairCd Antigen Cd340 antibody pairERBB2 antibody pairHER2 antibody pairMLN19 antibody pairNEU antibody pairNGL antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ErbB2/HER2 (ErbB2/HER2) Antibody Pair (STJAP00849)
This antibody pair detects human ErbB2/HER2, corresponding to UniProt entry P04626. Reactivity is confirmed for human samples only.
This antibody pair is validated for use in ELISA. It is intended for sandwich ELISA configurations using one antibody as capture and the other as detection.
Both antibodies are rabbit monoclonal IgGs, with clone IDs R03-2Z-3 and R09-6Y-5, raised against a recombinant protein immunogen. They are supplied unconjugated and affinity purified, formulated in 0.01M PBS at pH 7.2.
Optimal working concentrations for both the capture and detection antibodies should be determined by the end user through titration experiments. No predefined dilution range is specified for this product.
Both antibodies should be stored at 2 to 8 degrees Celsius. Freezing is not recommended.
No. This product is for research use only and is not intended for diagnostic, therapeutic, or clinical procedures.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.