Human EPO (Erythropoietin) Antibody Pair (STJAP00425)
General Information
| Short Description | This EPO antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | EPO |
| Gene ID | 2056 |
| Uniprot ID | EPO_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human EPO protien.Detection Immunogen: Recombinant Human EPO protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. |
| Protein Name | ErythropoietinEpoetin |
| Database Links | Reactome: R-HSA-1234158Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Erythropoietin antibody pairEpoetin antibody pairEPO antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human EPO (Erythropoietin) Antibody Pair (STJAP00425)
This antibody pair detects human Erythropoietin (EPO), corresponding to UniProt entry P01588. Reactivity is confirmed for human samples only.
Both antibodies are supplied at 0.5 mg/mL. The capture antibody is unconjugated and purified by Protein A or G in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is biotin-conjugated, purified by antigen affinity, and formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair is validated for use in ELISA. It is intended for sandwich ELISA development using the unconjugated antibody as the capture component and the biotinylated antibody as the detection component.
This antibody pair has been indicated for use with serum, plasma, and other biological fluids. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both the capture and detection antibodies were raised against recombinant human EPO protein. The capture antibody is hosted in mouse and the detection antibody in rabbit, based on the listed host species.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.