Human Eph receptor A2 (Eph receptor A2) Antibody Pair (STJAP00866)
General Information
| Short Description | This Eph receptor A2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R03-6T-2/R07-7C-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EPHA2 |
| Gene ID | 1969 |
| Uniprot ID | EPHA2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary. |
| Post Translational Modifications | Autophosphorylates. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-930 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-897 by PKB.serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Phosphorylated at Ser-897 in response to TNF by RPS6KA1 and RPS6KA3.RPS6KA-EPHA2 signaling pathway controls cell migration. Phosphorylated at Ser-897 by PKA.blocks cell retraction induced by EPHA2 kinase activity. Dephosphorylated by ACP1. Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation. |
| Function | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. Acts as a receptor for human cytomegalovirus (HCMV) to mediate viral entry and fusion in glioblastoma cells. |
| Protein Name | Ephrin Type-A Receptor 2Epithelial Cell KinaseTyrosine-Protein Kinase Receptor Eck |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-9013420Reactome: R-HSA-9013423Reactome: R-HSA-9013424Reactome: R-HSA-9696264Reactome: R-HSA-9696270Reactome: R-HSA-9696273 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionRuffle MembraneLamellipodium MembraneCell JunctionFocal AdhesionPresent At Regions Of Cell-Cell Contacts But Also At The Leading Edge Of Migrating CellsRelocates From The Plasma Membrane To The Cytoplasmic And Perinuclear Regions In Cancer Cells |
| Alternative Antibody Pair Names | Ephrin Type-A Receptor 2 antibody pairEpithelial Cell Kinase antibody pairTyrosine-Protein Kinase Receptor Eck antibody pairEPHA2 antibody pairECK antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Eph receptor A2 (Eph receptor A2) Antibody Pair (STJAP00866)
This antibody pair is designed to detect human Eph receptor A2 (UniProt P29317) and has confirmed reactivity against human samples only. No cross-reactivity data for other species is provided.
This pair is validated for use in ELISA. No other assay formats are listed as supported applications.
Both antibodies are rabbit monoclonal IgGs (clone IDs R03-6T-2 and R07-7C-5) supplied unconjugated and affinity purified, formulated in 0.01M PBS at pH 7.2. Neither antibody carries an enzyme or fluorescent conjugate, so end users are responsible for labeling the detection antibody as required for their assay setup.
Optimal working concentrations are determined by the end user and are not specified in the product documentation. Titration experiments are recommended to establish appropriate concentrations for each antibody in the intended assay format.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Both antibodies are rabbit monoclonal IgGs raised against a recombinant protein immunogen and purified by affinity chromatography. Specific purity percentages and immunogen region details are not provided in the current product documentation.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.