| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human EpCAM-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability of immobilized recombinant Human Cathepsin V to bind recombinant human EpCAM within a linear range of 10-100ng/ml in ELISA. NA |
| Gene Symbol: |
EPCAM |
| Gene ID: |
4072 |
| Uniprot ID: |
EPCAM_HUMAN |
| Immunogen Region: |
GLN24-LYS265, ECD |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Human Epithelial cell adhesion molecule (EpCAM) (GLN24-LYS265) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. |
| Post Translational Modifications | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Protein Name | Epithelial Cell Adhesion MoleculeEp-CamAdenocarcinoma-Associated AntigenCell Surface Glycoprotein Trop-1Epithelial Cell Surface AntigenEpithelial GlycoproteinEgpEpithelial Glycoprotein 314Egp314Hegp314Ks 1/4 AntigenKsaMajor Gastrointestinal Tumor-Associated Protein Ga733-2Tumor-Associated Calcium Signal Transducer 1Cd Antigen Cd326 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Lateral Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionTight JunctionColocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction |
| Alternative Protein Names | Epithelial Cell Adhesion Molecule proteinEp-Cam proteinAdenocarcinoma-Associated Antigen proteinCell Surface Glycoprotein Trop-1 proteinEpithelial Cell Surface Antigen proteinEpithelial GlycoproteinEgp proteinEpithelial Glycoprotein 314 proteinEgp314 proteinHegp314 proteinKs 1/4 Antigen proteinKsa proteinMajor Gastrointestinal Tumor-Associated Protein Ga733-2 proteinTumor-Associated Calcium Signal Transducer 1 proteinCd Antigen Cd326 proteinEPCAM proteinGA733-2 proteinM1S2 proteinM4S1 proteinMIC18 proteinTACSTD1 proteinTROP1 protein |
Information sourced from Uniprot.org
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