Human ENPP2 (Autotaxin) Antibody Pair (STJAP00391)
General Information
| Short Description | This ENPP2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | ENPP2 |
| Gene ID | 5168 |
| Uniprot ID | ENPP2_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ENPP2 protien.Detection Immunogen: Recombinant Human ENPP2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity. |
| Function | Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Its major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). |
| Protein Name | AutotaxinEctonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2E-Npp 2Extracellular Lysophospholipase DLysopld |
| Database Links | Reactome: R-HSA-199220 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Autotaxin antibody pairEctonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 antibody pairE-Npp 2 antibody pairExtracellular Lysophospholipase D antibody pairLysopld antibody pairENPP2 antibody pairATX antibody pairPDNP2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ENPP2 (Autotaxin) Antibody Pair (STJAP00391)
This antibody pair detects human ENPP2, also known as Autotaxin (UniProt Q13822). Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotinylated. Both are raised in goat, purified by antigen affinity, and supplied at 0.5 mg/mL.
This pair has been validated for use in ELISA. It is suitable for measuring ENPP2 in serum, plasma, and other biological fluids.
Both the capture and detection antibodies were raised against recombinant human ENPP2 protein. Both components were purified by antigen affinity chromatography.
Both antibodies should be stored at -20°C for long-term storage, with freeze-thaw cycles avoided. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.