• Human ENPP-1 protein (Recombinant) (C-His) (STJP002478)

Human ENPP-1 protein (Recombinant) (C-His) (STJP002478)

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STJP002478

£97.50 - £367.50
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Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 0.01 EU/Mu g
Immunoreactivity: Measured by its ability to hydrolyze thymidine 5 NA-monophosphate p-nitrophenyl ester. The specific activity is >63600 pmol/min/Mu g, as measured under the described conditions.
Gene Symbol: ENPP1
Gene ID: 5167
Uniprot ID: ENPP1_HUMAN
Immunogen Region: Lys98-Asp925
Immunogen: Recombinant Human ENPP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys98-Asp925) of human ENPP-1 (Accession #NP_006199.2) fused with a 6×His tag at the C-terminus.
Immunogen Sequence: KPSCAKEVKSCKGRCFERTF GNCRCDAACVELGNCCLDYQ ETCIEPEHIWTCNKFRCGEK RLTRSLCACSDDCKDKGDCC INYSSVCQGEKSWVEEPCES INEPQCPAGFETPPTLLFSL DGFRAEYLHTWGGLLPVISK LKKCGTYTKNMRPVYPTKTF PNHYSIVTGLYPESHGIIDN KMYDPKMNASFSLKSKEKFN PEWYKGEPIWVTAKYQGLKS GTFFWPGSDVEINGIFPDI
Post Translational Modifications Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity. N-glycosylated. The secreted form is produced through cleavage at Lys-103 by intracellular processing.
Function Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling.it is however unclear whether hedgehog inhibition is direct or indirect. Appears to modulate insulin sensitivity and function. Also involved in melanogenesis. Also able to hydrolyze 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production. 2',3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced.the role of 2',3'-cGAMP hydrolysis is therefore unclear. Not able to hydrolyze the 2',3'-cGAMP linkage isomer 3'-3'-cGAMP.
Protein Name Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1
E-Npp 1
Alkaline Phosphodiesterase I
Membrane Component Chromosome 6 Surface Marker 1
Nucleotide Diphosphatase
Nucleotide Pyrophosphatase
Nppase
Phosphodiesterase I/Nucleotide Pyrophosphatase 1
Plasma-Cell Membrane Glycoprotein Pc-1 Cleaved Into - Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1 - Secreted Form
Database Links Reactome: R-HSA-196843
Reactome: R-HSA-199220
Cellular Localisation Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1: Cell Membrane
Single-Pass Type Ii Membrane Protein
Basolateral Cell Membrane
Targeted To The Basolateral Membrane In Polarized Epithelial Cells And In Hepatocytes
And To Matrix Vesicles In Osteoblasts
In Bile Duct Cells And Cancer Cells
Located To The Apical Cytoplasmic Side
Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1
Secreted Form: Secreted
Secreted Following Proteolytic Cleavage
Alternative Protein Names Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1 protein
E-Npp 1 protein
Alkaline Phosphodiesterase I protein
Membrane Component Chromosome 6 Surface Marker 1 protein
Nucleotide Diphosphatase protein
Nucleotide Pyrophosphatase protein
Nppase protein
Phosphodiesterase I/Nucleotide Pyrophosphatase 1 protein
Plasma-Cell Membrane Glycoprotein Pc-1 Cleaved Into - Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1 - Secreted Form protein
ENPP1 protein
M6S1 protein
NPPS protein
PC1 protein
PDNP1 protein

Information sourced from Uniprot.org

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