Human ENO2 (Gamma-enolase) CLIA Kit for Quantitative Detection

Name: Human ENO2 (Gamma-enolase) CLIA Kit for Quantitative Detection
SKU: STJC0000499
Availability: InStock

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SPECIFICATIONS

STJC0000499

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Short Description	This human ENO2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	0.28ng/mL
Detection Limit	0.47~30ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	ENO2
Gene ID	2026
Uniprot ID	ENOG_HUMAN
Specificity	This kit recognizes Human NSE in samples. No significant cross-reactivity or interference between Human NSE and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Function	Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Protein Name	Gamma-Enolase 2-Phospho-D-Glycerate Hydro-Lyase Enolase 2 Neural Enolase Neuron-Specific Enolase Nse
Database Links	Reactome: R-HSA-70171 Reactome: R-HSA-70263
Cellular Localisation	Cytoplasm Cell Membrane Can Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) Form
Alternative CLIA Names	Gamma-Enolase CLIA kit 2-Phospho-D-Glycerate Hydro-Lyase CLIA kit Enolase 2 CLIA kit Neural Enolase CLIA kit Neuron-Specific Enolase CLIA kit Nse CLIA kit ENO2 CLIA kit
Specificity	This kit recognizes Human NSE in samples. No significant cross-reactivity or interference between Human NSE and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.49	4.37	10.89	1.35	4.66	11.50
Standard deviation	0.19	0.40	1.21	0.17	0.49	0.70
CV (%)	12.75	9.15	11.11	12.59	10.52	6.09