Human ENO1 (ENO1) Antibody Pair (STJAP00664)
General Information
| Short Description | This ENO1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 9F10/6H2 |
| Isotype | IgG2/IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ENO1 |
| Gene ID | 2023 |
| Uniprot ID | ENOA_HUMAN |
| Immunogen | A synthesized peptide derived from ENO1 |
Additional Info
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Post Translational Modifications | ISGylated. Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity. |
| Function | Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Isoform MBP-1: Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. |
| Protein Name | Alpha-Enolase2-Phospho-D-Glycerate Hydro-LyaseC-Myc Promoter-Binding ProteinEnolase 1Mbp-1Mpb-1Non-Neural EnolaseNnePhosphopyruvate HydratasePlasminogen-Binding Protein |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263Reactome: R-HSA-9636667 |
| Cellular Localisation | CytoplasmCell MembraneMyofibrilSarcomereM LineCan Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) FormEno1 Is Localized To The M LineIsoform Mbp-1: Nucleus |
| Alternative Antibody Pair Names | Alpha-Enolase antibody pair2-Phospho-D-Glycerate Hydro-Lyase antibody pairC-Myc Promoter-Binding Protein antibody pairEnolase 1 antibody pairMbp-1 antibody pairMpb-1 antibody pairNon-Neural Enolase antibody pairNne antibody pairPhosphopyruvate Hydratase antibody pairPlasminogen-Binding Protein antibody pairENO1 antibody pairENO1L1 antibody pairMBPB1 antibody pairMPB1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ENO1 (ENO1) Antibody Pair (STJAP00664)
This antibody pair detects human ENO1 (Alpha-enolase, UniProt P06733) and has confirmed reactivity to human samples only. It is not validated for ENO1 from other species.
Both antibodies are mouse monoclonal IgGs supplied as affinity-purified liquids at 1 mg/mL. The capture antibody (clone 9F10, IgG2) is formulated in PBS with 0.09% sodium azide, and the detection antibody (clone 6H2, IgG1) is formulated in PBS with 0.01% thimerosal. Neither antibody carries a conjugation or tag.
This antibody pair is validated for ELISA only, at a recommended dilution of 1:1000. It has not been reported as validated for other assay formats such as lateral flow, Western blot, or immunohistochemistry.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to preserve antibody integrity.
Both antibodies were raised against a synthesized peptide derived from human ENO1. No specific immunogen region data is provided in the product details.
No. This product is for research use only and is not approved or validated for diagnostic, therapeutic, or clinical applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.