Human Endothelial lipase protein (Recombinant) (STJP001873)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding peptidase domain of Human Endothelial lipase (SER21-PRO500) including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Endothelial lipase-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
Target Information
| Gene Symbol | LIPG |
| Gene ID | 9388 |
| Uniprot ID | LIPG_HUMAN |
| Immunogen | Optimized DNA sequence encoding peptidase domain of Human Endothelial lipase (SER21-PRO500) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | SER21-PRO500 |
Additional Info
| Tissue Specificity | High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not detected in heart, brain and muscle. |
| Function | Exerts both phospholipase and triglyceride lipase activities. More active as a phospholipase than a triglyceride lipase. Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. |
| Protein Name | Endothelial LipaseEndothelial Cell-Derived LipaseEdlElPhospholipase A1 |
| Database Links | Reactome: R-HSA-8964058 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Endothelial Lipase proteinEndothelial Cell-Derived Lipase proteinEdl proteinEl proteinPhospholipase A1 proteinLIPG proteinUNQ387 proteinPRO719 protein |
Information sourced from Uniprot.org