Human Endostatin (Endostatin) Antibody Pair (STJAP00648)
General Information
| Short Description | This Endostatin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 12G3/5E3 |
| Isotype | IgG1/IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | COL18A1 |
| Gene ID | 80781 |
| Uniprot ID | COIA1_HUMAN |
| Immunogen | A synthesized peptide derived from endostatin |
Additional Info
| Tissue Specificity | Detected in placenta (at protein level). Present in multiple organs with highest levels in liver, lung and kidney. |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated. Circulating endostatins are found as sialoglycoprotein and asialoglycoprotein structures. Undergoes proteolytic processing by CTSL/cathepsin-L and elastase-like proteases to generate both non-collagenous domain 1 trimers and endostatin monomers. In tissue extracts (brain, skeletal muscle, heart, kidney, testis and liver) predominantly bands of approximately 38 kDa are detected.recombinant non-collagenous domain 1 shows similar mobility. In vitro, several proteolytic cleavage sites in the non-collagenous domain 1 hinge region generating different endostatin-like peptides are reported. |
| Function | Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Non-collagenous domain 1: May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells.the function requires homotrimerization and implicates MAPK signaling. Endostatin: Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5. May negatively regulate the activity of homotrimeric non-collagenous domain 1. |
| Protein Name | Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1Nc1 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1592389Reactome: R-HSA-1650814Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-3000157Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneNon-Collagenous Domain 1: SecretedEndostatin: Secreted |
| Alternative Antibody Pair Names | Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1 antibody pairNc1 antibody pairCOL18A1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Endostatin (Endostatin) Antibody Pair (STJAP00648)
This antibody pair detects human Endostatin, corresponding to UniProt entry P39060. Reactivity has been confirmed for human samples only.
The pair consists of two mouse monoclonal antibodies: clone 12G3 (IgG1) and clone 5E3 (IgG2a). Both are supplied as unconjugated, affinity-purified antibodies at 1 mg/mL.
This pair is validated for use in sandwich ELISA at a dilution of 1:1000. No other assay formats are specified for this product.
The two antibodies differ in their preservative systems: one is formulated in PBS containing 0.09% sodium azide, and the other is formulated in PBS containing 0.01% thimerosal. Researchers should account for these preservatives when designing downstream assay conditions, as sodium azide in particular can interfere with enzyme-based detection systems.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies were raised against a synthesized peptide derived from human Endostatin. No further detail on the immunogen region is provided in the product specification.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.