Human EIF6 (Eukaryotic Translation Initiation Factor 6) Sandwich ELISA Kit (STJE0008609)
SPECIFICATIONS
General Information
| Short Description | This EIF6 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.123ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | EIF6 |
| Gene ID | 3692 |
| Uniprot ID | IF6_HUMAN |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export. Ufmylated by UFL1. |
| Function | Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth. |
| Protein Name | Eukaryotic Translation Initiation Factor 6Eif-6B(2gcn HomologB4 Integrin InteractorCabP27(Bbp |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusNucleolusShuttles Between Cytoplasm And Nucleus/Nucleolus |
| Alternative ELISA Names | Eukaryotic Translation Initiation Factor 6 ELISA kitEif-6 ELISA kitB(2gcn Homolog ELISA kitB4 Integrin Interactor ELISA kitCab ELISA kitP27(Bbp ELISA kitEIF6 ELISA kitEIF3A ELISA kitITGB4BP ELISA kitOK ELISA kitSW-cl.27 ELISA kit |
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Information sourced from Uniprot.org