Human eIF-5A2/EIF5A2 protein (Recombinant) (His-Tag) (STJP016736)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human eIF-5A2/EIF5A2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF5A2 |
| Gene ID | 56648 |
| Uniprot ID | IF5A2_HUMAN |
| Accession Number | NP_065123 |
| Immunogen | Human |
| Immunogen Region | 1-153aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells. |
| Post Translational Modifications | Lys-50 undergoes hypusination, a unique post-translational modification that consists in the addition of a butylamino group from spermidine to lysine side chain and leads to the formation of a hypusine residue. eIF-5As are the only known proteins to undergo this modification, which is essential for their function. |
| Function | Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step. Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. |
| Protein Name | Eukaryotic Translation Initiation Factor 5a-2Eif-5a-2Eif-5a2Eukaryotic Initiation Factor 5a Isoform 2 |
| Database Links | Reactome: R-HSA-204626 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideHypusine Modification Promotes The Nuclear Export And Cytoplasmic Localization And There Was A Dynamic Shift In The Localization From Predominantly Cytoplasmic To Primarily Nuclear Under Apoptotic Inducing Conditions |
| Alternative Protein Names | Eukaryotic Translation Initiation Factor 5a-2 proteinEif-5a-2 proteinEif-5a2 proteinEukaryotic Initiation Factor 5a Isoform 2 proteinEIF5A2 protein |
Information sourced from Uniprot.org