Human eIF-3 epsilon/EIF3F protein (Recombinant) (His-Tag) (STJP018460)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human eIF-3 epsilon/EIF3F-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Denatured |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 0.4M urea |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF3F |
| Gene ID | 8665 |
| Uniprot ID | EIF3F_HUMAN |
| Accession Number | NP_003745 |
| Immunogen | Human |
| Immunogen Region | 1-357aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit FEif3fDeubiquitinating Enzyme Eif3fEukaryotic Translation Initiation Factor 3 Subunit 5Eif-3-EpsilonEif3 P47 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Eukaryotic Translation Initiation Factor 3 Subunit F proteinEif3f proteinDeubiquitinating Enzyme Eif3f proteinEukaryotic Translation Initiation Factor 3 Subunit 5 proteinEif-3-Epsilon proteinEif3 P47 proteinEIF3F proteinEIF3S5 protein |
Information sourced from Uniprot.org