Human eIF-1AY/EIF1AY protein (Recombinant) (His-Tag) (STJP017040)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human eIF-1AY/EIF1AY-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100mM NaCl, 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF1AY |
| Gene ID | 9086 |
| Uniprot ID | IF1AY_HUMAN |
| Accession Number | NP_004672 |
| Immunogen | Human |
| Immunogen Region | 1-144aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1. |
| Protein Name | Eukaryotic Translation Initiation Factor 1a - Y-ChromosomalEif-1a Y IsoformEif1a Y IsoformEukaryotic Translation Initiation Factor 4cEif-4c |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Eukaryotic Translation Initiation Factor 1a - Y-Chromosomal proteinEif-1a Y Isoform proteinEif1a Y Isoform proteinEukaryotic Translation Initiation Factor 4c proteinEif-4c proteinEIF1AY protein |
Information sourced from Uniprot.org