Human EGFR2 (Receptor Tyrosine-Protein Kinase Erbb-2) Antibody Pair (STJAP00428)
General Information
| Short Description | This EGFR2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | ERBB2 |
| Gene ID | 2064 |
| Uniprot ID | ERBB2_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human EGFR2 protien.Detection Immunogen: Recombinant Human EGFR2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12. |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. |
| Protein Name | Receptor Tyrosine-Protein Kinase Erbb-2Metastatic Lymph Node Gene 19 ProteinMln 19Proto-Oncogene NeuProto-Oncogene C-Erbb-2Tyrosine Kinase-Type Cell Surface Receptor Her2P185erbb2Cd Antigen Cd340 |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-1306955Reactome: R-HSA-1358803Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-2219530Reactome: R-HSA-416572Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8863795Reactome: R-HSA-8866910Reactome: R-HSA-9634285Reactome: R-HSA-9652282Reactome: R-HSA-9664565Reactome: R-HSA-9665233Reactome: R-HSA-9665244Reactome: R-HSA-9665245Reactome: R-HSA-9665246Reactome: R-HSA-9665247Reactome: R-HSA-9665249Reactome: R-HSA-9665250Reactome: R-HSA-9665251Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9665737 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionRuffle MembraneInternalized From The Cell Membrane In Response To Egf StimulationIsoform 1: Cell MembraneEarly EndosomeCytoplasmPerinuclear RegionNucleusTranslocation To The Nucleus Requires EndocytosisProbably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1Also Detected In Vps35-Positive Endosome-To-Tgn Retrograde VesiclesIsoform 2: CytoplasmIsoform 3: Cytoplasm |
| Alternative Antibody Pair Names | Receptor Tyrosine-Protein Kinase Erbb-2 antibody pairMetastatic Lymph Node Gene 19 Protein antibody pairMln 19 antibody pairProto-Oncogene Neu antibody pairProto-Oncogene C-Erbb-2 antibody pairTyrosine Kinase-Type Cell Surface Receptor Her2 antibody pairP185erbb2 antibody pairCd Antigen Cd340 antibody pairERBB2 antibody pairHER2 antibody pairMLN19 antibody pairNEU antibody pairNGL antibody pair |
Information sourced from Uniprot.org
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Product Review
Frequently Asked Questions
Common questions about Human EGFR2 (Receptor Tyrosine-Protein Kinase Erbb-2) Antibody Pair (STJAP00428)
This antibody pair detects human EGFR2 (Receptor Tyrosine-Protein Kinase ErbB-2, UniProt P04626) and has confirmed reactivity with human samples. Both the capture and detection antibodies were raised against recombinant human EGFR2 protein.
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), while the detection antibody is biotin-conjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5). Both antibodies are rabbit-derived and purified by Protein A or G.
This pair has been validated for use in ELISA. It is intended for the detection of human EGFR2 in serum, plasma, and other biological fluids in a research setting.
The product has been validated for use with serum, plasma, and other biological fluids. This product is for research use only and is not intended for diagnostic or therapeutic applications.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.