| Host: | HEK293 cells |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Active Recombinant-Human EGF-C-hFc&His protein was developed from hek293 cells and has a target region of C-hFc&His. For use in research applications. |
| Formulation: | Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Immunoreactivity: | Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.2. Measured by its binding ability in a functional ELISA. Immobilized Hum |
| Gene Symbol: | EGF |
| Gene ID: | 1950 |
| Uniprot ID: | EGF_HUMAN |
| Immunogen Region: | Asn 971-Arg 1023 |
| Immunogen: | Recombinant Human EGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn 971-Arg 1023) of human EGF (Accession #NP_001954.2) fused with hFc, 6×His tag at the C-terminus. |
| Tissue Specificity | Expressed in kidney, salivary gland, cerebrum and prostate. |
| Post Translational Modifications | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Protein Name | Pro-Epidermal Growth FactorEgf Cleaved Into - Epidermal Growth FactorUrogastrone |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5637810Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9013507Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Pro-Epidermal Growth Factor proteinEgf Cleaved Into - Epidermal Growth Factor proteinUrogastrone proteinEGF protein |
Information sourced from Uniprot.org
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