Human EGF (Pro-epidermal growth factor) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human EGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 2.34pg/mL |
| Detection Limit | 3.91~250pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | EGF |
| Gene ID | 1950 |
| Uniprot ID | EGF_HUMAN |
| Specificity | This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in kidney, salivary gland, cerebrum and prostate. |
| Post Translational Modifications | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Protein Name | Pro-Epidermal Growth FactorEgf Cleaved Into - Epidermal Growth FactorUrogastrone |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5637810Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9013507Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9725554Reactome: R-HSA-9818030Reactome: R-HSA-9925561 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Pro-Epidermal Growth Factor CLIA kitEgf Cleaved Into - Epidermal Growth Factor CLIA kitUrogastrone CLIA kitEGF CLIA kit |
| Specificity | This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 101-115 | 107 |
| EDTA plasma(n=8) | 97-111 | 104 |
| Cell culture media(n=8) | 95-112 | 103 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 13.31 | 23.69 | 113.46 | 13.89 | 24.67 | 112.58 |
| Standard deviation | 1.21 | 2.08 | 8.82 | 1.43 | 1.81 | 9.96 |
| CV (%) | 9.09 | 8.78 | 7.77 | 10.30 | 7.34 | 8.85 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human EGF (Pro-epidermal growth factor) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect Human EGF (Pro-epidermal growth factor) in human serum, plasma, and other biological fluids. It is validated for human samples only and shows no significant cross-reactivity with analogues.
This kit has a detection range of 3.91 to 250 pg/mL and a sensitivity of 2.34 pg/mL. Samples with EGF concentrations outside the upper limit of the detection range should be diluted prior to testing.
This kit uses chemiluminescence (CLIA) as its detection method, which enables quantitative measurement of Human EGF in biological fluid samples.
The total assay time for this kit is approximately 3.5 hours.
If unopened, the kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month, components should be stored separately according to the component storage table provided in the manual.
According to the product specification, no significant cross-reactivity or interference between Human EGF and its analogues was observed with this kit.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.