Human EGF (EGF) Antibody Pair (STJAP00678)
General Information
| Short Description | This EGF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 5E9-A10-A6/7C11-G10-H8 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EGF |
| Gene ID | 1950 |
| Uniprot ID | EGF_HUMAN |
| Immunogen | A synthesized peptide derived from EGF |
Additional Info
| Tissue Specificity | Expressed in kidney, salivary gland, cerebrum and prostate. |
| Post Translational Modifications | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Protein Name | Pro-Epidermal Growth FactorEgf Cleaved Into - Epidermal Growth FactorUrogastrone |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5637810Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9013507Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9725554Reactome: R-HSA-9818030Reactome: R-HSA-9925561 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Pro-Epidermal Growth Factor antibody pairEgf Cleaved Into - Epidermal Growth Factor antibody pairUrogastrone antibody pairEGF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human EGF (EGF) Antibody Pair (STJAP00678)
This antibody pair detects human Epidermal Growth Factor (EGF), corresponding to UniProt entry P01133. Reactivity has been confirmed for human samples only.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies are mouse monoclonal IgG1 antibodies supplied as unconjugated, affinity-purified liquid at 1 mg/mL each. The capture antibody clone is 5E9-A10-A6 and the detection antibody clone is 7C11-G10-H8. Both are formulated in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide at pH 7.3.
A dilution of 1:1000 is recommended for ELISA applications. Researchers should perform optimization experiments to determine the most suitable working concentration for their specific experimental conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Both antibodies were raised against a synthesized peptide derived from human EGF. No additional immunogen region details are specified for this product.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.