Human EED protein (Recombinant) (His-Tag) (STJP018261)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human EED-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EED |
| Gene ID | 8726 |
| Uniprot ID | EED_HUMAN |
| Accession Number | NP_003788.2 |
| Immunogen | Human |
| Immunogen Region | 1-441aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. |
| Post Translational Modifications | Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines. |
| Function | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
| Protein Name | Polycomb Protein EedHeedEmbryonic Ectoderm Development ProteinWd Protein Associating With Integrin Cytoplasmic Tails 1Wait-1 |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-2559580Reactome: R-HSA-3214841Reactome: R-HSA-5617472Reactome: R-HSA-8943724Reactome: R-HSA-8953750Reactome: R-HSA-9609690Reactome: R-HSA-9710421 |
| Cellular Localisation | NucleusChromosomeTransiently Colocalizes With Xist At Inactive X Chromosomes |
| Alternative Protein Names | Polycomb Protein Eed proteinHeed proteinEmbryonic Ectoderm Development Protein proteinWd Protein Associating With Integrin Cytoplasmic Tails 1 proteinWait-1 proteinEED protein |
Information sourced from Uniprot.org