Human EDN1 (Endothelin-1) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human EDN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 4.69pg/mL |
| Detection Limit | 7.81~500pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | EDN1 |
| Gene ID | 1906 |
| Uniprot ID | EDN1_HUMAN |
| Specificity | This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2. |
| Protein Name | Endothelin-1Preproendothelin-1Ppet1 Cleaved Into - Endothelin-1Et-1 - Big Endothelin-1 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-9856649 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Endothelin-1 CLIA kitPreproendothelin-1 CLIA kitPpet1 Cleaved Into - Endothelin-1 CLIA kitEt-1 - Big Endothelin-1 CLIA kitEDN1 CLIA kit |
| Specificity | This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 102-117 | 108 |
| EDTA plasma(n=8) | 96-107 | 101 |
| Cell culture media(n=8) | 101-115 | 106 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 22.86 | 62.35 | 206.66 | 23.83 | 67.80 | 198.69 |
| Standard deviation | 2.46 | 6.53 | 20.48 | 2.29 | 7.92 | 16.23 |
| CV (%) | 10.76 | 10.47 | 9.91 | 9.61 | 11.68 | 8.17 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human EDN1 (Endothelin-1) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect Human Endothelin-1 (ET-1) in serum, plasma, and other biological fluids. It is reactive with human samples only.
This kit has a detection range of 7.81 to 500 pg/mL and a sensitivity of 4.69 pg/mL.
This kit uses chemiluminescence (CLIA) as the detection method for quantitative measurement of Human ET-1.
The total assay time is approximately 3.5 hours from start to finish.
If unopened, this kit may be stored at 2 to 8°C for up to one month. If the kit will not be used within one month, components should be stored separately according to the component table provided in the manual.
No significant cross-reactivity or interference between Human ET-1 and its analogues was observed, according to the specificity data for this kit.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.