Human ECHS1 protein (Recombinant) (N-His) (STJP004518)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ECHS1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ECHS1 |
| Gene ID | 1892 |
| Uniprot ID | ECHM_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ala28-Gln290 |
Additional Info
| Function | Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16. Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate. Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA). |
| Protein Name | Enoyl-Coa Hydratase - MitochondrialMechMech1Enoyl-Coa Hydratase 1Echs1Short-Chain Enoyl-Coa HydrataseSceh |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-77310Reactome: R-HSA-77346Reactome: R-HSA-77348Reactome: R-HSA-77350Reactome: R-HSA-77352Reactome: R-HSA-9916720 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Protein Names | Enoyl-Coa Hydratase - Mitochondrial proteinMech proteinMech1 proteinEnoyl-Coa Hydratase 1 proteinEchs1 proteinShort-Chain Enoyl-Coa Hydratase proteinSceh proteinECHS1 protein |
Information sourced from Uniprot.org