Human DTD2 protein (Recombinant) (His-Tag) (STJP018607)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human DTD2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DTD2 |
| Gene ID | 112487 |
| Uniprot ID | DTD2_HUMAN |
| Accession Number | NP_542395.1 |
| Immunogen | Human |
| Immunogen Region | 1-168aa |
| Immunogen Sequence |
Additional Info
| Function | Deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS. Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation. |
| Protein Name | D-Aminoacyl-Trna Deacylase 2Animalia-Specific Trna DeacylaseAtdD-Tyrosyl-Trna(Tyr Deacylase 2L-Alanyl-Trna Deacylase |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | D-Aminoacyl-Trna Deacylase 2 proteinAnimalia-Specific Trna Deacylase proteinAtd proteinD-Tyrosyl-Trna(Tyr Deacylase 2 proteinL-Alanyl-Trna Deacylase proteinDTD2 proteinC14orf126 protein |
Information sourced from Uniprot.org