Human DPH3 protein (Recombinant) (C-His) (STJP014765)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human DPH3-C-His protein was developed from e.coli and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | DPH3 |
| Gene ID | 285381 |
| Uniprot ID | DPH3_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Cys82 |
Additional Info
| Function | Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Acts as an electron donor to reduce the Fe-S cluster in DPH1-DPH2 keeping the 4Fe-4S clusters in the active and reduced state. Restores iron to DPH1-DPH2 iron-sulfur clusters which have degraded from 4Fe-4S to 3Fe-4S by donating an iron atom to reform 4Fe-4S clusters, in a manner dependent on the presence of elongation factor 2 and SAM. Associates with the elongator complex and is required for tRNA Wobble base modifications mediated by the elongator complex. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). |
| Protein Name | Diphthamide Biosynthesis Protein 3Csl-Type Zinc Finger-Containing Protein 2Delgef-Interacting Protein 1Delgip1 |
| Database Links | Reactome: R-HSA-5358493 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | Diphthamide Biosynthesis Protein 3 proteinCsl-Type Zinc Finger-Containing Protein 2 proteinDelgef-Interacting Protein 1 proteinDelgip1 proteinDPH3 proteinDESR1 proteinZCSL2 protein |
Information sourced from Uniprot.org