Human Doublecortin (Doublecortin) Antibody Pair (STJAP00770)
General Information
| Short Description | This Doublecortin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R34-7M-3/R16-8A-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DCX |
| Gene ID | 1641 |
| Uniprot ID | DCX_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. |
| Post Translational Modifications | Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. |
| Function | Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. |
| Protein Name | Neuronal Migration Protein DoublecortinDoublinLissencephalin-XLis-X |
| Database Links | Reactome: R-HSA-447043 |
| Cellular Localisation | CytoplasmCell ProjectionNeuron ProjectionLocalizes At Neurite Tips |
| Alternative Antibody Pair Names | Neuronal Migration Protein Doublecortin antibody pairDoublin antibody pairLissencephalin-X antibody pairLis-X antibody pairDCX antibody pairDBCN antibody pairLISX antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Doublecortin (Doublecortin) Antibody Pair (STJAP00770)
This antibody pair is designed to detect human Doublecortin (UniProt O43602) and has confirmed reactivity against the human protein only.
This antibody pair has been validated for use in ELISA. It has not been validated for other assay formats based on the available product data.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. The clone IDs are R34-7M-3 and R16-8A-1 respectively.
Working concentrations for both the capture and detection antibodies are to be determined by the end user. Titration optimization is recommended when setting up the ELISA.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles.
Both antibodies in this pair were raised in rabbit using a recombinant protein immunogen corresponding to human Doublecortin.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.