Human DHCR7 (7-Dehydrocholesterol Reductase) Sandwich ELISA Kit (STJE0008424)
SPECIFICATIONS
General Information
| Short Description | This DHCR7 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.062ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | DHCR7 |
| Gene ID | 1717 |
| Uniprot ID | DHCR7_HUMAN |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Widely expressed. Most abundant in adrenal gland, liver, testis, and brain. |
| Post Translational Modifications | |
| Function | Oxidoreductase that catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core. Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol. Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation. Component of the microsomal antiestrogen binding site (AEBS), a multiproteic complex at the ER membrane that consists of an association between cholestenol Delta-isomerase/EBP and DHCR7. This complex is responsible for cholesterol-5,6-epoxide hydrolase (ChEH) activity, which consists in the hydration of cholesterol-5,6-epoxides (5,6-EC) into cholestane-3beta,5alpha,6beta-triol (CT). The precise role of each component of this complex has not been described yet. |
| Protein Name | 7-Dehydrocholesterol Reductase7-Dhc ReductaseCholesterol-5 -6-Epoxide Hydrolase Subunit Dhcr7Delta7-Sterol ReductaseSterol Delta(7-ReductaseSterol Reductase Sr-2 |
| Database Links | Reactome: R-HSA-2426168Reactome: R-HSA-6807047Reactome: R-HSA-6807062 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | 7-Dehydrocholesterol Reductase ELISA kit7-Dhc Reductase ELISA kitCholesterol-5 -6-Epoxide Hydrolase Subunit Dhcr7 ELISA kitDelta7-Sterol Reductase ELISA kitSterol Delta(7-Reductase ELISA kitSterol Reductase Sr-2 ELISA kitDHCR7 ELISA kitD7SR ELISA kit |
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Information sourced from Uniprot.org