Human DEFB4A; DEFB4B (Beta-defensin 4A) Sandwich ELISA Kit (STJE0002646)
SPECIFICATIONS
General Information
| Short Description | This DEFB4A; DEFB4B Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 37.5pg/mL |
| Detection Limit | 62.5~4000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8Β°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | DEFB4A.DEFB4B |
| Gene ID | 100289462/1673 |
| Uniprot ID | DFB4A_HUMAN |
| Specificity | This kit recognizes Human DEF Beta 2/DEFB2 in samples. No significant cross-reactivity or interference between Human DEF Beta 2/DEFB2 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria. Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM. Also exhibits antimicrobial activity against the yeast C.albicans. Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death. Acts as a ligand for C-C chemokine receptor CCR6. Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells. |
| Protein Name | Defensin Beta 4aBeta-Defensin 2Bd-2Hbd-2Defensin - Beta 2Skin-Antimicrobial Peptide 1Sap1 |
| Database Links | Reactome: R-HSA-1461957Reactome: R-HSA-1461973 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Defensin Beta 4a ELISA kitBeta-Defensin 2 ELISA kitBd-2 ELISA kitHbd-2 ELISA kitDefensin - Beta 2 ELISA kitSkin-Antimicrobial Peptide 1 ELISA kitSap1 ELISA kitDEFB4A ELISA kitDEFB102 ELISA kitDEFB2 ELISA kitDEFB4.DEFB4B ELISA kit |
| Specificity | This kit recognizes Human DEFΞ²2/DEFB2 in samples. No significant cross-reactivity or interference between Human DEFΞ²2/DEFB2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells Γ12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100Γ) | 96T: 1 vial, 120 ΞΌL 60 ΞΌL | -20℃, 6 months |
| Concentrated HRP Conjugate (100Γ) | 96T: 1 vial, 120 ΞΌL 60 ΞΌL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8Β°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8Β°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8Β°C, 6 months |
| Concentrated Wash Buffer (25Γ) | 1 vial, 30 mL | 2-8Β°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8Β°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-100 | 94 |
| EDTA plasma (n=8) | 86-97 | 91 |
| Cell culture media (n=8) | 88-102 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 182.36 | 364.62 | 1818.71 | 180.04 | 341.12 | 1735.26 |
| Standard deviation | 12.73 | 17.76 | 88.39 | 12.46 | 17.50 | 57.26 |
| CV (%) | 6.98 | 4.87 | 4.86 | 6.92 | 5.13 | 3.30 |