Human DEFB1 (Beta-defensin 1) Sandwich ELISA Kit (STJE0002645)

Name: Human DEFB1 (Beta-defensin 1) Sandwich ELISA Kit (STJE0002645)
SKU: STJE0002645
Availability: InStock

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SPECIFICATIONS

STJE0002645

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Short Description	This DEFB1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	DEFB1
Gene ID	1672
Uniprot ID	DEFB1_HUMAN
Specificity	This kit recognizes Human DEFB1 in samples. No significant cross-reactivity or interference between Human DEFB1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
Protein Name	Beta-Defensin 1 Bd-1 Hbd-1 Defensin - Beta 1
Database Links	Reactome: R-HSA-1461957 Reactome: R-HSA-1461973
Cellular Localisation	Secreted Membrane Associates With Tumor Cell Membrane-Derived Microvesicles
Alternative ELISA Names	Beta-Defensin 1 ELISA kit Bd-1 ELISA kit Hbd-1 ELISA kit Defensin - Beta 1 ELISA kit DEFB1 ELISA kit BD1 ELISA kit HBD1 ELISA kit
Specificity	This kit recognizes Human DEFB1 in samples. No significant cross-reactivity or interference between Human DEFB1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	95.42	291.69	820.81	101.98	320.07	801.87
Standard deviation	5.20	17.03	41.45	6.37	13.96	38.09
CV (%)	5.45	5.84	5.05	6.25	4.36	4.75