Human DEF Beta 2/DEFB2 (Defensin Beta 4a) Antibody Pair (STJAP00420)
General Information
| Short Description | This DEF Beta 2/DEFB2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | DEFB4A.DEFB4B |
| Gene ID | 100289462/1673 |
| Uniprot ID | DFB4A_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human DEF Beta 2/DEFB2 protien.Detection Immunogen: Recombinant Human DEF Beta 2/DEFB2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria. Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM. Also exhibits antimicrobial activity against the yeast C.albicans. Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death. Acts as a ligand for C-C chemokine receptor CCR6. Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells. |
| Protein Name | Defensin Beta 4aBeta-Defensin 2Bd-2Hbd-2Defensin - Beta 2Skin-Antimicrobial Peptide 1Sap1 |
| Database Links | Reactome: R-HSA-1461957Reactome: R-HSA-1461973 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Defensin Beta 4a antibody pairBeta-Defensin 2 antibody pairBd-2 antibody pairHbd-2 antibody pairDefensin - Beta 2 antibody pairSkin-Antimicrobial Peptide 1 antibody pairSap1 antibody pairDEFB4A antibody pairDEFB102 antibody pairDEFB2 antibody pairDEFB4.DEFB4B antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human DEF Beta 2/DEFB2 (Defensin Beta 4a) Antibody Pair (STJAP00420)
This antibody pair detects Human DEF Beta 2 (DEFB2, also known as Defensin Beta 4a), corresponding to UniProt entry O15263. Reactivity is confirmed for human samples only.
Both antibodies are polyclonal, raised in goat, and purified by antigen affinity chromatography. The capture antibody is unconjugated, and the detection antibody is biotin-conjugated. Both are supplied at 0.5 mg/mL.
This pair is validated for use in ELISA. It has not been listed as validated for other assay formats such as lateral flow or multiplex-based assays.
The capture antibody is formulated in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS containing 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair is intended for use with human serum, plasma, and other biological fluids. It is intended for research use only and is not validated for diagnostic or clinical applications.
Both antibodies should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.